Recombinant Platelet Derived Growth Factor D (PDGFD)

Organism Species: Homo sapiens (Human)

[ PROPERTIES ]

NUMBER:  YB919Hu01

Source:  Prokaryotic expression

Host:  E.coli

Residues:  Arg19~Arg370

Tags:  Two N-terminal Tags, His-tag and SUMO-tag

Subcellular Location:  Secreted

Purity:  > 85%

Traits:  Freeze-dried powder

Buffer formulation:  PBS, PH7.4, pH8.0, containing 0.01% skl, 5%Trehalose.

Original Concentration:  220µg/mL

Applications:  Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:  8.4

Predicted Molecular Mass:  54.4kDa

Accurate Molecular Mass:  48kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

               Store at 2-8ºC for one month.

               Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure . Gene Sequencing (extract)

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.