Rabbit Anti-3-Nitrotyrosine Polyclonal Antibody

Host:Rabbit 

Size:100ul

Target Protein:3-Nitrotyrosine 

IR:Immunogen Range: 

Clonality:Polyclonal 

Isotype:IgG 

Source:KLH conjugated Nitrotyrosine: 

Purification:affinity purified by Protein A 

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

Background:Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as theformation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage invivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure. Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as animportant component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to becapable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited tospecific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, tooxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-Aat tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis. 

Concentration:1mg/ml 

Applications:WB(1:500-2000)、ELISA(1:5000-10000)、IHC-P(1:100-500)、IHC-F(1:100-500)、Flow-Cyt(1μg/Test)、IF(1:50-200)Cross Reactive Species:Human、Mouse、Rat、Nitrotyrosine. 

For research use only. Not intended for diagnostic or therapeutic